Proteomic Analysis of Proteins Secreted from Hepatocytes
نویسندگان
چکیده
منابع مشابه
Proteomic analysis of secreted proteins from Arabidopsis thaliana seedlings: improved recovery following removal of phenolic compounds.
Arabidopsis thaliana seedlings grown in liquid culture were used to recover proteins secreted from the whole plant. The aim was to identify apoplastic proteins that may be lost during classical extraction procedures such as preparation of cell walls. The inclusion of polyvinyl-polypyrrolidone (PVPP) in the protocol of purification of secreted proteins allowed a more efficient identification of ...
متن کاملAnalysis of secreted proteins from Aspergillus flavus.
MS/MS techniques in proteomics make possible the identification of proteins from organisms with little or no genome sequence information available. Peptide sequences are obtained from tandem mass spectra by matching peptide mass and fragmentation information to protein sequence information from related organisms, including unannotated genome sequence data. This peptide identification data can t...
متن کاملComparative proteomic analysis of extracellular matrix proteins secreted by hypertrophic scar with normal skin fibroblasts
The formation of hypertrophic scars (HSs) is a fibroproliferative disorder of abnormal wound healing. HSs usually characterize excessive proliferation of fibroblasts, abnormal deposition of extracellular matrix (ECM) during wound healing, associated with cosmetic, functional, and psychological problems. Owing to the role of ECM proteins in scar formation, we comparatively analyzed matrix protei...
متن کاملProteomic analysis of cartilage proteins.
While the analysis of the cartilage proteome is important for our comprehensive understanding of the development and disease of this important tissue, several unique features of cartilage present some technical obstacles. Firstly, cartilage is difficult to obtain in adequate quantities for many protein analyses, especially from mice which are otherwise powerful experimental models. Furthermore,...
متن کاملTop-Down proteomic analysis of CSF proteins from ALS patients
ALS has largely unknown pathogenesis that typically result in death within a few years from diagnosis [1]. There are currently no effective therapies for ALS. Clinical diagnosis usually takes several months to complete and the long delay between symptom onset and diagnosis, limits the possibilities for effective intervention and clinical trials [2]. The establishment of protein biomarkers for A...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Mass Spectrometry Society of Japan
سال: 2005
ISSN: 1340-8097,1880-4225
DOI: 10.5702/massspec.53.164